Use of denaturing gradient gel electrophoresis in screening unknown β-thalassemia mutations in Egyptian patients
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چکیده
منابع مشابه
Identification of the multiple beta-thalassemia mutations by denaturing gradient gel electrophoresis.
We used denaturing gradient gel electrophoresis to detect the beta-thalassemia mutations in the Chinese population. By amplifying the beta-globin gene in four separate fragments and electrophoresing the amplified DNA in two gels, we were able to distinguish all the 12 known mutations on the basis of the mobility of the homoduplexes and heteroduplexes. We conclude that denaturing gradient gel el...
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A large number of hprt-mutants were obtained by treating human lymphoblast cells (TK6) with 5 microM K2Cr2O7 for 5 hr and selecting by growth in 6-thioguanine. A combination of high fidelity polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) allowed us to measure mutant frequencies as a function of DNA sequence. Chromium(VI) induced four hotspots in a 104 bp doma...
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Bacteria play an important role in the initiation and progression of diseases (not only in disease but affect health status of individual). Populations of digestive microflora in chicken gut change with age and also affected by diet, stressors, and performance enhancers as well as in disease conditions. The aim of the pre-sent study was to know bacterial makeup in chicken gut, using denaturing ...
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References 1. Davis HG, Hagen PO. Systemic inflammatory response syndrome. Br J Surg 1997;84:920–35. 2. Fraser WD, Taggert DP, Fell GS, Lyon TDB, Wheatley D, Garden OJ, Shenkin A. Changes in iron, zinc and copper concentrations in serum and in their binding to transport proteins after cholecystectomy and cardiac surgery. Clin Chem 1990;35:2243–7. 3. Hawker FH, Stewart PM, Snitch PJ. Effect of a...
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Single-strand conformational polymorphism and denaturing gel electrophoresis were used to screen for mutations in the protoporphyrinogen oxidase gene (PPOX) of three patients with clinically and biochemically proven variegate porphyria in order to select genomic regions for specific DNA sequence analysis. Two previously undescribed mutations were identified: PPOX1423-1426-delATCT and PPOX2272in...
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ژورنال
عنوان ژورنال: Egyptian Journal of Medical Human Genetics
سال: 2012
ISSN: 1110-8630
DOI: 10.1016/j.ejmhg.2012.06.008